LOCUS       AMC-941_Htr2a-EGFP-CT-I 8830 bp ds-DNA     circular     16-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    67..1027
                     /label="5HR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            285..1027
                     /label="Ex3-5'"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    895..994
                     /label="genotype region 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1028..1047
                     /label="SENSE_PRM"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1028..1048
                     /label="Linker"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1049..1765
                     /label="EGFP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1049..1765
                     /label="Translation 1049-1765"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     misc_feature    complement(1145..1244)
                     /label="genotype region 2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1766..1789
                     /label="Linker"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            1790..1849
                     /label="Ex3-3'"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1847..1849
                     /label="TGA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1874..2437
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2435..5359
                     /label="ECE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5185..5284
                     /label="genotype region 3"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5366..5413
                     /label="FRT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     3'UTR           5422..5883
                     /label="Htr2a\3'UTR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 5450..5450
                     /label="G->C"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5451..6681
                     /label="3HR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(5520..5620)
                     /label="genotype region 4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   6355..6589
                     /label="Repeat_Region_194"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     repeat_region   complement(6432..6459)
                     /label="Repeat_Region_195"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    6729..6871
                     /label="SV40pA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(7044..7717)
                     /label="pUC\Ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(7862..8722)
                     /label="Amp(R)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        complement(8723..8792)
                     /label="Pbla"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 atttccccga aaagtgccac ctggtcgaca ttgattattg actagtgcga attggagctc
       61 cccgggtttg tttcactttg cctctgcatg tgtttttgcc agttaataag taaaaaataa
      121 tataattcga aaaaatatag aataagaatt agcccaatct tcattgaagt aatactctta
      181 aagtaagttg gcatcacttt cttgaccagg cattaagtgg tgatgcttgt accttcatag
      241 tctcagtccc ttgatggtct gtctcacttt tcatcctttt tcaggtatat ccatgccaat
      301 cccagtcttc gggctacagg atgattcgaa ggtctttaag gaagggagct gcctgctcgc
      361 cgatgacaac tttgtcctca taggctcttt tgtggcattt ttcatccccc taaccatcat
      421 ggtgatcacc tacttcctga ctatcaagtc acttcagaaa gaagccacct tgtgtgtgag
      481 tgacctcagc actcgggcca aattatcctc cttcagcttc ctccctcaga gttctctgtc
      541 atcagaaaag ctcttccagc ggtccatcca cagagagcca ggctcctacg caggccgaag
      601 gacgatgcag tccatcagca acgagcaaaa agcatgcaag gtgctgggca tcgtgttctt
      661 tctgtttgtt gtaatgtggt gcccattctt catcaccaat atcatggccg tcatctgcaa
      721 agaatcctgc aatgaaaatg tcattggagc cctgctcaat gtgtttgtct ggattggtta
      781 tctctcctca gccgtcaacc cactggtata tacgttgttc aataaaactt ataggtccgc
      841 cttctcacgg tacattcagt gccagtacaa ggagaacaga aagccgctgc agttaatttt
      901 agtgaacact ataccaacat tggcctacaa gtctagtcag ctccaggtgg gacaaaaaaa
      961 gaactcacag gaagatgctg agccgacagc taatgactgc tccatggtta cactagggaa
     1021 ccaacacggt tcaggtggag gcagcggcat ggtgtccaag ggcgaggagc tgttcaccgg
     1081 ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc
     1141 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     1201 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     1261 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1321 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1381 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1441 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1501 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1561 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1621 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     1681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1741 tcacggcatg gacgagctgt acaagggagg ctcaggggga ggctcaggat cggaagagat
     1801 gtgtacagac aatattgaaa ccgtgaatga aaaggttagc tgtgtgtgaa aactacgggc
     1861 tgcaggaatt ccgccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1921 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1981 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     2041 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     2101 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     2161 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     2221 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     2281 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     2341 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     2401 gacgtggttt tcctttgaaa aacacgatga taatatggct ggagacatga gagctgccaa
     2461 cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct
     2521 gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga
     2581 gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc
     2641 agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt
     2701 gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg
     2761 tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt
     2821 ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc
     2881 tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc
     2941 tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga
     3001 agagaaggac catatccacc gagtcctgga caagatcaca gacactttga tccacctgat
     3061 ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat
     3121 cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg
     3181 caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca
     3241 tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac
     3301 tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg
     3361 tttccctgcc acagctgtcg acaatttact gaccgtacac caaaatttgc ctgcattacc
     3421 ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
     3481 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg
     3541 gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct
     3601 tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct
     3661 aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact
     3721 ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct
     3781 agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
     3841 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc
     3901 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat
     3961 ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct
     4021 gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa
     4081 taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca
     4141 gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc
     4201 taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc
     4261 cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg
     4321 gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat
     4381 ggtgcgcctg ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa
     4441 cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct
     4501 gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga
     4561 gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc
     4621 agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt
     4681 gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg
     4741 tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt
     4801 ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc
     4861 tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc
     4921 tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga
     4981 agagaaggac catatccacc gagtcctgga caagatcaca gacactttga tccacctgat
     5041 ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat
     5101 cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg
     5161 caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca
     5221 tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac
     5281 tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg
     5341 tttccctgcc acagcttgaa caattgaagt tcctattccg aagttcctat tctctagaaa
     5401 gtataggaac ttcgcggccg ctggaccgga tgctgggacg attgcccagc gtgtgtgaac
     5461 aagtttacac ccacgtgtgt gggggcagga taaggaggct cagatcaaat tagactactc
     5521 cagtggacca actataatgt ccggacagca tttgaactag gagtgttgtg atgcttttaa
     5581 cattgccaat gagatcttta aaatcattgg cctttattgt acaattgtaa tgaggcataa
     5641 aatccatcta attaacttct attgtcaaat agaaaccggg ctgccatgtt gttgatgggt
     5701 agcatgggag taagttggtg acatattgtt gtatataaaa atagctataa atactgaaaa
     5761 taatgaatag cctctttaaa aacaaaaatg agtgtttcaa attcaccaca acatatattt
     5821 tgaaaagaaa aatagacagt attatgacat ttgtattgct aatacaattc attgaaatac
     5881 ttgacgatat ttttcattct tgcttttttc atagatgcca ttttgaaatg ttcacaagac
     5941 cgctggcatt tgctgcattt gtcattaatt ctcagacgtg gaagggtctt aaatgttatt
     6001 caataactcg ctgctttctc ttctacttct tgggctttac ctgaatttgc aatgtggtct
     6061 tatttcgtat atttttcttc tctgtaaact atcaaagggt aattctactt ttccaagtac
     6121 agttctagaa ctcgcttcaa agaaacagca tcccggaggt atttggtaac tcactaggga
     6181 aatgactgtg ttgcgcatgc gccctttgag cagtgaccag tacaaagagt tgactgtcac
     6241 agctgaacat taaactcagg tttctcgctg ttagcagtag cttcttaggt tatttctgta
     6301 aaactcaagt gactttcata ttgacagtta tcaggtaaaa ctggtgtttt caaagagctg
     6361 gcaacgtagc ccagttgata aaagagtgct tgcctaacat gcaggaaatc ctagtgtgga
     6421 tgcccaacac tacataacat aacataacat aacataacag gcaccacggt gcatggctgt
     6481 aatcctagca cttaggaagt gcaggcgaga ggatcaggaa ttcagtcacc ctcagctatt
     6541 tatctagtat gaggccaatg tggactgcat gagagactct gtctccaaac taaataaata
     6601 tagggttttt taatgatact tttagattaa tgtatttata ttatttataa aaatgggtta
     6661 gcttggttcc acagtgatct gccgcgggga gctccaattc gcacgcgtcc taggcttttg
     6721 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6781 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6841 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     6901 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7081 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7261 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     7321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7501 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7801 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7861 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7921 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7981 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8041 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8101 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8161 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8221 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8281 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8341 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8401 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8461 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8521 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8581 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8641 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8701 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8761 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8821 ttccgcgcac
//