LOCUS AMC-1009_Htr2a-IRES-Cre 6196 bp ds-DNA circular 31-JUL-2020 DEFINITION . KEYWORDS "creator:Dale Cowley" "marker:Htr2a 3'UTR, 3HR" COMMENT Donor Vector for AMC-1009 designed to insert IRES-Cre-FRT at stop codon or Htr2a gene. Note 3HR has a G->C point mutation designed to inactivate a gRNA in the AMC-941 project from which the vector was derived. FEATURES Location/Qualifiers misc_feature 67..1087 /label="5HR2" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" exon 285..1087 /label=3 /ApEinfo_revcolor="#708090" /ApEinfo_fwdcolor="#708090" misc_feature 973..1072 /label="genotype region 1" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" misc_feature 1083..1087 /label="sg84T" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 1084..1087 /label="sg77T" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 1085..1087 /label="TGA" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 1112..1675 /label="IRES" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 1136..1235 /label="genotype region 2" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" CDS 1676..2722 /label="NLS-Cre" /ApEinfo_revcolor="#993366" /ApEinfo_fwdcolor="#993366" misc_feature 2651..2724 /label="genotype region 3" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 2723..2725 /label="stop" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" protein_bind 2732..2779 /label="FRT" /ApEinfo_revcolor="#31849b" /ApEinfo_fwdcolor="#31849b" 3'UTR 2788..3249 /label="Htr2a 3'UTR" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 2792..2862 /label="genotype region 4" /ApEinfo_revcolor="#d6b295" /ApEinfo_fwdcolor="#d6b295" misc_difference 2816..2816 /label="G->C" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" misc_feature 2817..4047 /label="3HR" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" repeat_region 3721..3955 /label="Repeat_Region_194" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" repeat_region 3798..3825 /label="Repeat_Region_195" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" polyA_signal 4095..4237 /label="SV40pA" /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" rep_origin complement(4410..5083) /label="pUC Ori" /ApEinfo_revcolor="#ffef86" /ApEinfo_fwdcolor="#ffef86" CDS complement(5228..6088) /label="Amp(R)" /ApEinfo_revcolor="#993366" /ApEinfo_fwdcolor="#993366" promoter complement(6089..6158) /label="Pbla" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" ORIGIN 1 atttccccga aaagtgccac ctggtcgaca ttgattattg actAGTGCGA ATTGGAGCTC 61 CCCGGGTTTG TTTCACTTTG CCTCTGCatg tgtttttgcc agttaataag taaaaaataa 121 tataattcga aaaaatatag aataagaatt agcccaatct tcattgaagt aatactctta 181 aagtaagttg gcatcacttt cttgaccagg cattaagtgg tgatgcttgt accttcatag 241 tctcagtccc ttgatggtct gtctcacttt tcatcctttt tcaggtatat ccatgccaat 301 cccagtcttc gggctacagg atgattcgaa ggtctttaag gaagggagct gcctgctcgc 361 cgatgacaac tttgtcctca taggctcttt tgtggcattt ttcatccccc taaccatcat 421 ggtgatcacc tacttcctga ctatcaagtc acttcagaaa gaagccacct tgtgtgtgag 481 tgacctcagc actcgggcca aattatcctc cttcagcttc ctccctcaga gttctctgtc 541 atcagaaaag ctcttccagc ggtccatcca cagagagcca ggctcctacg caggccgaag 601 gacgatgcag tccatcagca acgagcaaaa agcatgcaag gtgctgggca tcgtgttctt 661 tctgtttgtt gtaatgtggt gcccattctt catcaccaat atcatggccg tcatctgcaa 721 agaatcctgc aatgaaaatg tcattggagc cctgctcaat gtgtttgtct ggattggtta 781 tctctcctca gccgtcaacc cactggtata tacgttgttc aataaaactt ataggtccgc 841 cttctcacgg tacattcagt gccagtacaa ggagaacaga aagccgctgc agttaatttt 901 agtgaacact ataccaacat tggcctacaa gtctagtcag ctccaggtgg gacaaaaaaa 961 gaactcacag gaagatgctg agccgacagc taatgactgc tccatggtta cactagggaa 1021 ccaacactcg gaagagatgt gtacagacaa tattgaaacc gtgaatgaaa aggttagctg 1081 tgtgtgaAAA CTACGGGCTG CAGGAATtcc gcccccccct aacgttactg gccgaagccg 1141 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 1201 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 1261 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 1321 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 1381 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 1441 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 1501 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 1561 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta 1621 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatgcccaa 1681 gaagaagagg aaggtgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt 1741 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc 1801 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg 1861 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct 1921 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa 1981 catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt 2041 tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc 2101 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca 2161 ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac gtatagccga 2221 aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca 2281 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg 2341 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa 2401 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct 2461 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa 2521 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc 2581 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac 2641 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt 2701 gcgccTGCTG GAAGATGGCG ATTAAATGCA TGAAGTTCCT ATTCCGAAGT TCCTATTCTC 2761 TAGAAAGTAT AGGAACTTCG CGGCCGCTGG ACCGGATGCT Gggacgattg cccagcgtgt 2821 gtgaacaagt ttacacccac gtgtgtgggg gcaggataag gaggctcaga tcaaattaga 2881 ctactccagt ggaccaacta taatgtccgg acagcatttg aactaggagt gttgtgatgc 2941 ttttaacatt gccaatgaga tctttaaaat cattggcctt tattgtacaa ttgtaatgag 3001 gcataaaatc catctaatta acttctattg tcaaatagaa accgggctgc catgttgttg 3061 atgggtagca tgggagtaag ttggtgacat attgttgtat ataaaaatag ctataaatac 3121 tgaaaataat gaatagcctc tttaaaaaca aaaatgagtg tttcaaattc accacaacat 3181 atattttgaa aagaaaaata gacagtatta tgacatttgt attgctaata caattcattg 3241 aaatacttga cgatattttt cattcttgct tttttcatag atgccatttt gaaatgttca 3301 caagaccgct ggcatttgct gcatttgtca ttaattctca gacgtggaag ggtcttaaat 3361 gttattcaat aactcgctgc tttctcttct acttcttggg ctttacctga atttgcaatg 3421 tggtcttatt tcgtatattt ttcttctctg taaactatca aagggtaatt ctacttttcc 3481 aagtacagtt ctagaactcg cttcaaagaa acagcatccc ggaggtattt ggtaactcac 3541 tagggaaatg actgtgttgc gcatgcgccc tttgagcagt gaccagtaca aagagttgac 3601 tgtcacagct gaacattaaa ctcaggtttc tcgctgttag cagtagcttc ttaggttatt 3661 tctgtaaaac tcaagtgact ttcatattga cagttatcag gtaaaactgg tgttttcaaa 3721 gagctggcaa cgtagcccag ttgataaaag agtgcttgcc taacatgcag gaaatcctag 3781 tgtggatgcc caacactaca taacataaca taacataaca taacaggcac cacggtgcat 3841 ggctgtaatc ctagcactta ggaagtgcag gcgagaggat caggaattca gtcaccctca 3901 gctatttatc tagtatgagg ccaatgtgga ctgcatgaga gactctgtct ccaaactaaa 3961 taaatatagg gttttttaat gatactttta gattaatgta tttatattat ttataaaaat 4021 gggttagctt ggttccacag tgatctgccg cggggagctc caattcgcac gcgtcctagg 4081 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4201 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg 4261 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 4321 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 4381 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 4441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 4501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 4561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 4621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 4681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 4861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 4921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 4981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 5041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 5161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 5221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 5281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 5341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 5401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 5461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 5521 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 5581 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 5641 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 5701 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 5761 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 5821 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 5881 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 5941 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 6001 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6061 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 6181 taggggttcc gcgcac //