LOCUS       AMC-1029-Htr2a-A        7830 bp    DNA     circular SYN 02-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2026 from SnapGene 8.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    67..1266
                     /label=1029-5HR
                     /note="color: #a6acb3"
     repeat_region   97..228
                     /label=Repeat_Region_189
                     /note="color: #a6acb3"
     repeat_region   248..269
                     /label=Repeat_Region_190
                     /note="color: #a6acb3"
     repeat_region   403..496
                     /label=Repeat_Region_191
                     /note="color: #a6acb3"
     repeat_region   729..738
                     /label=Repeat_Region_192
                     /note="color: #a6acb3"
     repeat_region   821..829
                     /label=Repeat_Region_193
                     /note="color: #a6acb3"
     CDS             1157..1899
                     /codon_start=3
                     /label=Ex3-5' with A242S
                     /note="color: #993366"
                     /translation="ISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIM
                     VITYFLTIKSLQKEATLCVSDLSTRAKLSSFSFLPQSSLSSEKLFQRSIHREPGSYAGR
                     RTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNENVIGALLNVFVWI
                     GYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENRKPLQLILVNTIPTLAYKSSQLQVG
                     QKKNSQEDAEPTANDCSMVTLGNQH"
     misc_feature    1246..1264
                     /label=g52T
                     /note="guide RNA site
                     "
                     /note="color: #a6acb3"
     misc_feature    1267..1269
                     /label=A242S
                     /note="color: #a6acb3"
     CDS             1900..1920
                     /label=Linker
                     /note="color: #993366; direction: RIGHT"
     CDS             1921..2637
                     /label=EGFP
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1989
                     /label=G-A Variant
                     /note="color: #a6acb3"
     misc_feature    2615
                     /label=H232L Variant
                     /note="color: #a6acb3"
     CDS             2638..2661
                     /label=Linker
                     /note="color: #993366; direction: RIGHT"
     CDS             2662..2721
                     /label=Ex3-3'
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2719..2721
                     /label=TGA
                     /note="color: #a6acb3"
     misc_feature    2746..3309
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             3310..4356
                     /label=NLS-Cre
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4357..4359
                     /label=stop
                     /note="color: #a6acb3"
     protein_bind    4366..4413
                     /label=FRT
                     /note="color: #31849b"
     3'UTR           4422..4883
                     /label=Htr2a 3'UTR
                     /note="color: #a6acb3"
     misc_difference 4450
                     /label=G->C
                     /note="color: #a6acb3"
     misc_feature    4451..5681
                     /label=3HR
                     /note="color: #a6acb3"
     repeat_region   5355..5589
                     /label=Repeat_Region_194
                     /note="color: #a6acb3"
     repeat_region   5432..5459
                     /label=Repeat_Region_195
                     /note="color: #a6acb3"
     polyA_signal    5729..5871
                     /label=SV40pA
                     /note="color: #a6acb3"
     rep_origin      complement(6044..6717)
                     /direction=LEFT
                     /label=pUC Ori
                     /note="color: #ffff00"
     CDS             complement(6862..7722)
                     /label=Amp(R)
                     /note="color: #993366; direction: LEFT"
     promoter        complement(7723..7792)
                     /label=Pbla
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 atttccccga aaagtgccac ctggtcgaca ttgattattg actagtgcga attggagctc
       61 cccgggtgat tgtgggtttc tctgaaagtg tttcacttta aatctacata agaatatgtg
      121 tacatgttta atcatatgat atctgaggtt tgcctcaaaa tgagggttcc aagtgagggc
      181 cctggaagac acaatatttg tcttgaattt ataattataa aagctaagag gcatccttgg
      241 gaaaccattt ttctttgttc gttttttttg gggggtttgc agatgtaaga atttttccaa
      301 agtaaaaact ttataaaact aacccaagtt catttgactt acttgaaatt aatattggaa
      361 aatgtctttc aaaagtagtt tagaatatgt tcaactttaa aaatggccat agtggctcat
      421 gtcattcaag aagctgaagc aggatcaatg tgagtctgag gttatcccag gctagtatca
      481 gaccctgtct caacaagcac acgttttatc ataaatactt aatgccatag ggagacctaa
      541 aactgcacag aaagttctgt gcaaacaaaa gtaacctagg caccttggtg gtgagaataa
      601 gtgcgatttt cctttactca ctgttacctt tttatatttt tcaaatttgt tagaaagcaa
      661 agatgttagg cttataagtt tacagaagaa gtcgtgaatg ctgatagtat aatatgaagt
      721 gggaagttaa aaaaaaaagt agataaacta gacccttgtc ctcttgccca gtgtgtgcac
      781 gagtacatat gtgtgtgagc gcgtgcgtgt gtgggtacgt ggggggggga gtacatgcgt
      841 gcatatgttc ctgcatgtgt catgtgtgtg atacatgtta aactgtccct gcggttagtc
      901 tgttgatctc tagatgatgg tgttagcggt catttgtatt tgtttcactt tgcctctgca
      961 tgtgtttttg ccagttaata agtaaaaaat aatataattc gaaaaaatat agaataagaa
     1021 ttagcccaat cttcattgaa gtaatactct taaagtaagt tggcatcact ttcttgacca
     1081 ggcattaagt ggtgatgctt gtaccttcat agtctcagtc ccttgatggt ctgtctcact
     1141 tttcatcctt tttcaggtat atccatgcca atcccagtct tcgggctaca ggatgattcg
     1201 aaggtcttta aggaagggag ctgcctgctc gccgatgaca actttgtcct cataggctct
     1261 tttgtgtcct ttttcatccc cctaaccatc atggtgatca cctacttcct gactatcaag
     1321 tcacttcaga aagaagccac cttgtgtgtg agtgacctca gcactcgggc caaattatcc
     1381 tccttcagct tcctccctca gagttctctg tcatcagaaa agctcttcca gcggtccatc
     1441 cacagagagc caggctccta cgcaggccga aggacgatgc agtccatcag caacgagcaa
     1501 aaagcatgca aggtgctggg catcgtgttc tttctgtttg ttgtaatgtg gtgcccattc
     1561 ttcatcacca atatcatggc cgtcatctgc aaagaatcct gcaatgaaaa tgtcattgga
     1621 gccctgctca atgtgtttgt ctggattggt tatctctcct cagccgtcaa cccactggta
     1681 tatacgttgt tcaataaaac ttataggtcc gccttctcac ggtacattca gtgccagtac
     1741 aaggagaaca gaaagccgct gcagttaatt ttagtgaaca ctataccaac attggcctac
     1801 aagtctagtc agctccaggt gggacaaaaa aagaactcac aggaagatgc tgagccgaca
     1861 gctaatgact gctccatggt tacactaggg aaccaacacg gttcaggtgg aggcagcggc
     1921 atggtgtcca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1981 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2161 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2341 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2401 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2461 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2521 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2581 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaaggga
     2641 ggctcagggg gaggctcagg atcggaagag atgtgtacag acaatattga aaccgtgaat
     2701 gaaaaggtta gctgtgtgtg aaaactacgg gctgcaggaa ttccgccccc ccctaacgtt
     2761 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     2821 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     2881 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     2941 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3001 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3061 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     3121 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     3181 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     3241 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     3301 gataatatgc ccaagaagaa gaggaaggtg tccaatttac tgaccgtaca ccaaaatttg
     3361 cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc
     3421 agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg
     3481 tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt
     3541 cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat
     3601 ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat
     3661 gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca
     3721 aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat
     3781 agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg
     3841 ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg
     3901 agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag
     3961 gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct
     4021 gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct
     4081 gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg
     4141 atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc
     4201 cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa
     4261 gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa
     4321 acaggggcaa tggtgcgcct gctggaagat ggcgattaaa tgcatgaagt tcctattccg
     4381 aagttcctat tctctagaaa gtataggaac ttcgcggccg ctggaccgga tgctgggacg
     4441 attgcccagc gtgtgtgaac aagtttacac ccacgtgtgt gggggcagga taaggaggct
     4501 cagatcaaat tagactactc cagtggacca actataatgt ccggacagca tttgaactag
     4561 gagtgttgtg atgcttttaa cattgccaat gagatcttta aaatcattgg cctttattgt
     4621 acaattgtaa tgaggcataa aatccatcta attaacttct attgtcaaat agaaaccggg
     4681 ctgccatgtt gttgatgggt agcatgggag taagttggtg acatattgtt gtatataaaa
     4741 atagctataa atactgaaaa taatgaatag cctctttaaa aacaaaaatg agtgtttcaa
     4801 attcaccaca acatatattt tgaaaagaaa aatagacagt attatgacat ttgtattgct
     4861 aatacaattc attgaaatac ttgacgatat ttttcattct tgcttttttc atagatgcca
     4921 ttttgaaatg ttcacaagac cgctggcatt tgctgcattt gtcattaatt ctcagacgtg
     4981 gaagggtctt aaatgttatt caataactcg ctgctttctc ttctacttct tgggctttac
     5041 ctgaatttgc aatgtggtct tatttcgtat atttttcttc tctgtaaact atcaaagggt
     5101 aattctactt ttccaagtac agttctagaa ctcgcttcaa agaaacagca tcccggaggt
     5161 atttggtaac tcactaggga aatgactgtg ttgcgcatgc gccctttgag cagtgaccag
     5221 tacaaagagt tgactgtcac agctgaacat taaactcagg tttctcgctg ttagcagtag
     5281 cttcttaggt tatttctgta aaactcaagt gactttcata ttgacagtta tcaggtaaaa
     5341 ctggtgtttt caaagagctg gcaacgtagc ccagttgata aaagagtgct tgcctaacat
     5401 gcaggaaatc ctagtgtgga tgcccaacac tacataacat aacataacat aacataacag
     5461 gcaccacggt gcatggctgt aatcctagca cttaggaagt gcaggcgaga ggatcaggaa
     5521 ttcagtcacc ctcagctatt tatctagtat gaggccaatg tggactgcat gagagactct
     5581 gtctccaaac taaataaata tagggttttt taatgatact tttagattaa tgtatttata
     5641 ttatttataa aaatgggtta gcttggttcc acagtgatct gccgcgggga gctccaattc
     5701 gcacgcgtcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7801 gaaaaataaa caaatagggg ttccgcgcac
//
