LOCUS AMC-1029-Htr2a-A 7830 bp DNA circular SYN 02-DEC-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7830) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 13, 2026 from SnapGene 8.2.0 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7830 /mol_type="other DNA" /note="color: #ffffff" /organism="synthetic DNA construct" misc_feature 67..1266 /label=1029-5HR /note="color: #a6acb3" repeat_region 97..228 /label=Repeat_Region_189 /note="color: #a6acb3" repeat_region 248..269 /label=Repeat_Region_190 /note="color: #a6acb3" repeat_region 403..496 /label=Repeat_Region_191 /note="color: #a6acb3" repeat_region 729..738 /label=Repeat_Region_192 /note="color: #a6acb3" repeat_region 821..829 /label=Repeat_Region_193 /note="color: #a6acb3" CDS 1157..1899 /codon_start=3 /label=Ex3-5' with A242S /note="color: #993366" /translation="ISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIM VITYFLTIKSLQKEATLCVSDLSTRAKLSSFSFLPQSSLSSEKLFQRSIHREPGSYAGR RTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNENVIGALLNVFVWI GYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENRKPLQLILVNTIPTLAYKSSQLQVG QKKNSQEDAEPTANDCSMVTLGNQH" misc_feature 1246..1264 /label=g52T /note="guide RNA site " /note="color: #a6acb3" misc_feature 1267..1269 /label=A242S /note="color: #a6acb3" CDS 1900..1920 /label=Linker /note="color: #993366; direction: RIGHT" CDS 1921..2637 /label=EGFP /note="color: #993366; direction: RIGHT" misc_feature 1989 /label=G-A Variant /note="color: #a6acb3" misc_feature 2615 /label=H232L Variant /note="color: #a6acb3" CDS 2638..2661 /label=Linker /note="color: #993366; direction: RIGHT" CDS 2662..2721 /label=Ex3-3' /note="color: #993366; direction: RIGHT" misc_feature 2719..2721 /label=TGA /note="color: #a6acb3" misc_feature 2746..3309 /label=IRES /note="color: #a6acb3" CDS 3310..4356 /label=NLS-Cre /note="color: #993366; direction: RIGHT" misc_feature 4357..4359 /label=stop /note="color: #a6acb3" protein_bind 4366..4413 /label=FRT /note="color: #31849b" 3'UTR 4422..4883 /label=Htr2a 3'UTR /note="color: #a6acb3" misc_difference 4450 /label=G->C /note="color: #a6acb3" misc_feature 4451..5681 /label=3HR /note="color: #a6acb3" repeat_region 5355..5589 /label=Repeat_Region_194 /note="color: #a6acb3" repeat_region 5432..5459 /label=Repeat_Region_195 /note="color: #a6acb3" polyA_signal 5729..5871 /label=SV40pA /note="color: #a6acb3" rep_origin complement(6044..6717) /direction=LEFT /label=pUC Ori /note="color: #ffff00" CDS complement(6862..7722) /label=Amp(R) /note="color: #993366; direction: LEFT" promoter complement(7723..7792) /label=Pbla /note="color: #ffffff; direction: LEFT" ORIGIN 1 atttccccga aaagtgccac ctggtcgaca ttgattattg actagtgcga attggagctc 61 cccgggtgat tgtgggtttc tctgaaagtg tttcacttta aatctacata agaatatgtg 121 tacatgttta atcatatgat atctgaggtt tgcctcaaaa tgagggttcc aagtgagggc 181 cctggaagac acaatatttg tcttgaattt ataattataa aagctaagag gcatccttgg 241 gaaaccattt ttctttgttc gttttttttg gggggtttgc agatgtaaga atttttccaa 301 agtaaaaact ttataaaact aacccaagtt catttgactt acttgaaatt aatattggaa 361 aatgtctttc aaaagtagtt tagaatatgt tcaactttaa aaatggccat agtggctcat 421 gtcattcaag aagctgaagc aggatcaatg tgagtctgag gttatcccag gctagtatca 481 gaccctgtct caacaagcac acgttttatc ataaatactt aatgccatag ggagacctaa 541 aactgcacag aaagttctgt gcaaacaaaa gtaacctagg caccttggtg gtgagaataa 601 gtgcgatttt cctttactca ctgttacctt tttatatttt tcaaatttgt tagaaagcaa 661 agatgttagg cttataagtt tacagaagaa gtcgtgaatg ctgatagtat aatatgaagt 721 gggaagttaa aaaaaaaagt agataaacta gacccttgtc ctcttgccca gtgtgtgcac 781 gagtacatat gtgtgtgagc gcgtgcgtgt gtgggtacgt ggggggggga gtacatgcgt 841 gcatatgttc ctgcatgtgt catgtgtgtg atacatgtta aactgtccct gcggttagtc 901 tgttgatctc tagatgatgg tgttagcggt catttgtatt tgtttcactt tgcctctgca 961 tgtgtttttg ccagttaata agtaaaaaat aatataattc gaaaaaatat agaataagaa 1021 ttagcccaat cttcattgaa gtaatactct taaagtaagt tggcatcact ttcttgacca 1081 ggcattaagt ggtgatgctt gtaccttcat agtctcagtc ccttgatggt ctgtctcact 1141 tttcatcctt tttcaggtat atccatgcca atcccagtct tcgggctaca ggatgattcg 1201 aaggtcttta aggaagggag ctgcctgctc gccgatgaca actttgtcct cataggctct 1261 tttgtgtcct ttttcatccc cctaaccatc atggtgatca cctacttcct gactatcaag 1321 tcacttcaga aagaagccac cttgtgtgtg agtgacctca gcactcgggc caaattatcc 1381 tccttcagct tcctccctca gagttctctg tcatcagaaa agctcttcca gcggtccatc 1441 cacagagagc caggctccta cgcaggccga aggacgatgc agtccatcag caacgagcaa 1501 aaagcatgca aggtgctggg catcgtgttc tttctgtttg ttgtaatgtg gtgcccattc 1561 ttcatcacca atatcatggc cgtcatctgc aaagaatcct gcaatgaaaa tgtcattgga 1621 gccctgctca atgtgtttgt ctggattggt tatctctcct cagccgtcaa cccactggta 1681 tatacgttgt tcaataaaac ttataggtcc gccttctcac ggtacattca gtgccagtac 1741 aaggagaaca gaaagccgct gcagttaatt ttagtgaaca ctataccaac attggcctac 1801 aagtctagtc agctccaggt gggacaaaaa aagaactcac aggaagatgc tgagccgaca 1861 gctaatgact gctccatggt tacactaggg aaccaacacg gttcaggtgg aggcagcggc 1921 atggtgtcca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1981 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 2041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 2101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 2161 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 2221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2341 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 2401 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 2461 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 2521 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 2581 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaaggga 2641 ggctcagggg gaggctcagg atcggaagag atgtgtacag acaatattga aaccgtgaat 2701 gaaaaggtta gctgtgtgtg aaaactacgg gctgcaggaa ttccgccccc ccctaacgtt 2761 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc 2821 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc 2881 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag 2941 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg 3001 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat 3061 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga 3121 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc 3181 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg 3241 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat 3301 gataatatgc ccaagaagaa gaggaaggtg tccaatttac tgaccgtaca ccaaaatttg 3361 cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc 3421 agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg 3481 tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt 3541 cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat 3601 ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat 3661 gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca 3721 aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat 3781 agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg 3841 ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg 3901 agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag 3961 gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct 4021 gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct 4081 gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg 4141 atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc 4201 cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa 4261 gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa 4321 acaggggcaa tggtgcgcct gctggaagat ggcgattaaa tgcatgaagt tcctattccg 4381 aagttcctat tctctagaaa gtataggaac ttcgcggccg ctggaccgga tgctgggacg 4441 attgcccagc gtgtgtgaac aagtttacac ccacgtgtgt gggggcagga taaggaggct 4501 cagatcaaat tagactactc cagtggacca actataatgt ccggacagca tttgaactag 4561 gagtgttgtg atgcttttaa cattgccaat gagatcttta aaatcattgg cctttattgt 4621 acaattgtaa tgaggcataa aatccatcta attaacttct attgtcaaat agaaaccggg 4681 ctgccatgtt gttgatgggt agcatgggag taagttggtg acatattgtt gtatataaaa 4741 atagctataa atactgaaaa taatgaatag cctctttaaa aacaaaaatg agtgtttcaa 4801 attcaccaca acatatattt tgaaaagaaa aatagacagt attatgacat ttgtattgct 4861 aatacaattc attgaaatac ttgacgatat ttttcattct tgcttttttc atagatgcca 4921 ttttgaaatg ttcacaagac cgctggcatt tgctgcattt gtcattaatt ctcagacgtg 4981 gaagggtctt aaatgttatt caataactcg ctgctttctc ttctacttct tgggctttac 5041 ctgaatttgc aatgtggtct tatttcgtat atttttcttc tctgtaaact atcaaagggt 5101 aattctactt ttccaagtac agttctagaa ctcgcttcaa agaaacagca tcccggaggt 5161 atttggtaac tcactaggga aatgactgtg ttgcgcatgc gccctttgag cagtgaccag 5221 tacaaagagt tgactgtcac agctgaacat taaactcagg tttctcgctg ttagcagtag 5281 cttcttaggt tatttctgta aaactcaagt gactttcata ttgacagtta tcaggtaaaa 5341 ctggtgtttt caaagagctg gcaacgtagc ccagttgata aaagagtgct tgcctaacat 5401 gcaggaaatc ctagtgtgga tgcccaacac tacataacat aacataacat aacataacag 5461 gcaccacggt gcatggctgt aatcctagca cttaggaagt gcaggcgaga ggatcaggaa 5521 ttcagtcacc ctcagctatt tatctagtat gaggccaatg tggactgcat gagagactct 5581 gtctccaaac taaataaata tagggttttt taatgatact tttagattaa tgtatttata 5641 ttatttataa aaatgggtta gcttggttcc acagtgatct gccgcgggga gctccaattc 5701 gcacgcgtcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca 5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat 5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7801 gaaaaataaa caaatagggg ttccgcgcac //